PE Mouse Anti-Human IFN-γ 

Clone  4S.B3   (RUO)

• BrandBD Pharmingen™

• Alternative NameIFNG; Interferon-gamma; IFG; IFI; Type II interferon

• Vol. Per Test20 µl

• IsotypeMouse BALB/c IgG1, κ

• ReactivityRhesus, Cynomolgus, Baboon (QC Testing)  Human (Tested in Development) 

• ApplicationIntracellular staining (flow cytometry) (Routinely Tested) 
  

• ImmunogenHuman IFN-γ from supernatants of S. aureus-stimulated PBMC

• Storage BufferAqueous buffered solution containing BSA and ≤0.09% sodium azide.

• Description

• The 4S.B3 monoclonal antibody specifically binds to interferon-γ (IFN-γ). The immunogen used to generate this hybridoma was partially purified human IFN-γ obtained from supernatants of human PBMC stimulated with Staphylococcus aureus. Interferon-γ (IFN-γ) is a potent multifunctional cytokine that is produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ Receptor Complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γ is a 14-18 kDa glycoprotein containing 143 amino acid residues.

• Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ + cells within a mixed cell population.

• FORMAT

• 

• FormatPE

• Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm

• Excitation Max496 nm

• Emission Max578 nm

• R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.