Alexa Fluor® 647 Mouse Anti-Human CD63  Clone  H5C6  (RUO)

• Brand

• BD Pharmingen™

• Alternative Name

• LAMP-3; ME491; MLA-1; Granulophysin; Ptgr40; NGA; gp55

• Vol. Per Test

• 5 µl

• Isotype

• Mouse BALB/c IgG1, κ

• Reactivity

• Human (QC Testing) 

• Application

• Flow cytometry (Routinely Tested) 
  

• Immunogen

• Human Splenic Adherent Cells

• Workshop No.

• V P036

• Storage Buffer

• Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

DESCRIPTION

The H5C6 monoclonal antibody reacts with CD63. CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on activated platelets, monocytes and macrophages. This molecule is also referred to in the literature as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a member of the tetraspan transmembrane 4 superfamily (TM4SF). It is widely expressed on surface and in the cytoplasm of various hematopoietic (monocytes, macrophages) and non-hematopoietic cells (endothelium, fibroblasts, osteoclasts, smooth muscle).

• ormat

• Alexa Fluor® 647

• Excitation Source

• Red 633 nm

• Excitation Max

• 650 nm

• Emission Max

• 668 nm

Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.